Zymogram Development Buffer [10X] (0.5M Tris.HCl, 2M NaCl, 50mM CaCl2, 0.2% Brij-35, pH 7.5) - 125 ml


Zymogram Development Buffer [10X] (0.5M Tris.HCl, 2M NaCl, 50mM CaCl2, 0.2% Brij-35, pH 7.5) - 125 ml by Cepham Life Sciences at Periodic Table on the Web. MPN: 10504. Hurry! Limited time offer. Offer valid only while supplies last. Our Zymogram Developing Buffer is used for Running Protein samples for Zymogram PAGE analysis on Polyacrylamide gels. The buffer is supplied as 10X concentration and contains 0.5 Tris HCl, 2M NaCl, 50mM CaCl2, 0.2% Brij-35, pH 7.5. Simply dilute it by adding 1/10 volume of 10X Zymogram Developing Buffer to 9/10 volume of DI Water.Zymography is an electrophoretic technique, which is based on SDS-PAGE and includes an enzyme substrate copolymerized with polyacrylamide gel.  Samples are prepared in


Zymogram Development Buffer [10X] (0.5M Tris.HCl, 2M NaCl, 50mM CaCl2, 0.2% Brij-35, pH 7.5) - 125 ml by Cepham Life Sciences
4.1 out of 5 stars with 14 reviews
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Availability: In Stock
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Product Description & Reviews

Our Zymogram Developing Buffer is used for Running Protein samples for Zymogram PAGE analysis on Polyacrylamide gels. The buffer is supplied as 10X concentration and contains 0.5 Tris HCl, 2M NaCl, 50mM CaCl2, 0.2% Brij-35, pH 7.5. Simply dilute it by adding 1/10 volume of 10X Zymogram Developing Buffer to 9/10 volume of DI Water.Zymography is an electrophoretic technique, which is based on SDS-PAGE and includes an enzyme substrate copolymerized with polyacrylamide gel.  Samples are prepared in Zymogram Sample Buffer without boiling to preserve the structure and activity of the enzyme. Following electrophoresis, the SDS is removed from the gel by washing in Zymogram Renature Buffer that contains a non-ionic detergent. The gels are then equilibrated in Zymogram Developing Buffer, which contains the divalent metal cation calcium, required to activate calcium-dependent proteases such as matrix metalloproteinases (MMPs). The zymogram is subsequently stained (commonly with Amido Black or Coomassie Brilliant Blue), and areas of digestion appear as clear bands against a darkly stained background where the substrate has been degraded by the enzyme.

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Additional Information

Brand:
Cepham Life Sciences
Manufacturer:
Cepham Life Sciences
Category:
Enzymes
MPN:
10504
Part Number:
10504
Publisher:
Cepham Life Sciences

 


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