Lysozyme


Lysozyme by Bio Basic at Periodic Table on the Web. MPN: LDB0308.SIZE.100g. Hurry! Limited time offer. Offer valid only while supplies last. Bio Basic’s Lysozyme Lysozyme is a single chain polypeptide of 129 amino acids cross-linked with four disulfide bridges. It hydrolyzes ß(1→4) linkages between N-acetylmuraminic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a


Lysozyme by Bio Basic
4.2 out of 5 stars with 14 reviews
Condition: New
Availability: In Stock
$358.00


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Product Description & Reviews

Bio Basic’s Lysozyme Lysozyme is a single chain polypeptide of 129 amino acids cross-linked with four disulfide bridges. It hydrolyzes ß(1→4) linkages between N-acetylmuraminic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gramnegative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed more easily in the presence of EDTA that chelates metal ions in the outer bacterial membrane. Source: This lysozyme preparation is purified from chicken egg white, crystallized three times, dialyzed, and supplied as a lyophilized powder. Protein content by UV absorbance is ≥90% with the remainder (~10%) being buffer salts such as sodium acetate and sodium chloride. Purity: This highly purified enzyme preparation has been used in mass spectrometry as a protein mass calibration standard and in structural studies of proteins. It is suitable for use as a lysing agent in the purification of plasmid DNA using a boiling lysing technique. ✔️ Molecular mass: 14,307 Da (amino acid sequence) ✔️ Isoelectric point (pI): 11.35 Optimal pH: The activity of lysozyme is a function of both pH and ionic strength. The enzyme is active over a broad pH range (6.0–9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02–0.100 M) than at pH 9.2 (0.01–0.06 M). Inhibitors:

Features & Highlights

  • ✔️ Molecular mass: 14,307 Da (amino acid sequence)
  • ✔️ Lysozyme activity: ≥20,000 units/mg protein
  • ✔️ Isoelectric point (pI): 11.35
  • ✔️ Store at -20 °C for long term use.

Additional Information

Brand:
Bio Basic
Manufacturer:
Bio Basic
Category:
Enzymes
MPN:
LDB0308.SIZE.100g
Part Number:
LDB0308.SIZE.100g
Publisher:
Bio Basic
Item Weight:
0.22 pounds
Item Size:
0.02 x 0.02 x 0.035 inches

 


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